1MQJ

Crystal structure of the GluR2 ligand binding core (S1S2J) in complex with willardiine at 1.65 angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	110
Detector technology	CCD
Collection date	2000-04-11
Detector	ADSC QUANTUM 4
Wavelength(s)	1.100
Spacegroup name	P 21 21 2
Unit cell lengths	64.142, 93.223, 48.537
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.650
R-factor	0.208
Rwork	0.208
R-free	0.22900
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1mqi
RMSD bond length	0.006
RMSD bond angle	1.220 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.055	0.279
Number of reflections	35502
Completeness [%]	99.2	93.7
Redundancy	5.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	4 *	PEG 5K MME, Na Citrate, zinc acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mM)
2	1	reservoir	PEG5000 MME	14-18 (%)
3	1	reservoir	zinc acetate	0.1 (M)
4	1	reservoir	sodium citrate	0.1 (M)	pH5.5