1MKK
Disulfide deficient mutant of vascular endothelial growth factor A (C61A and C104A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8423 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.220, 75.470, 55.120 |
| Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.320 |
| R-factor | 0.16836 |
| Rwork | 0.165 |
| R-free | 0.19515 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vpf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.350 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.074 | 0.375 |
| Number of reflections | 53321 | |
| <I/σ(I)> | 12.08 | 2.48 |
| Completeness [%] | 99.2 | 99.5 |
| Redundancy | 3.8 | 3.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | isopropanol, PEG4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | sodium citrate | 0.05 (M) | |
| 5 | 1 | drop | 2-propanol | 6 (%(v/v)) | |
| 6 | 1 | drop | PEG4000 | 14 (%) | |
| 7 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 8 | 1 | reservoir | 2-propanol | 12 (%(v/v)) | |
| 9 | 1 | reservoir | PEG4000 | 28 (%) |
