1MKG
DISULFIDE DEFICIENT MUTANT OF VASCULAR ENDOTHELIAL GROWTH FACTOR A (C57A and C102A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-08-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8423 |
Spacegroup name | P 65 |
Unit cell lengths | 96.200, 96.200, 125.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.000 - 2.500 |
R-factor | 0.243 |
Rwork | 0.238 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vpf |
RMSD bond length | 0.011 |
RMSD bond angle | 1.510 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.066 | 0.400 |
Number of reflections | 22584 | |
<I/σ(I)> | 14.84 | 2.92 |
Completeness [%] | 98.6 | 99 |
Redundancy | 4.7 | 4.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | Na-formate 2.0 M, Na-acetate 0.1 M, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |