1MJN
Crystal Structure of the intermediate affinity aL I domain mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2000-12-17 |
| Detector | SBC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.374, 57.399, 66.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.300 |
| Rwork | 0.156 |
| R-free | 0.20000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lfa |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.088 | 0.352 |
| Total number of observations | 197616 * | |
| Number of reflections | 35329 * | |
| <I/σ(I)> | 17.8 | 2.4 |
| Completeness [%] | 90.2 | 47 |
| Redundancy | 5.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30 (%) | |
| 3 | 1 | reservoir | 0.05 (M) | ||
| 4 | 1 | reservoir | Tris-Cl | 0.1 (M) | pH8.5 |
