1MIZ
Crystal structure of an integrin beta3-talin chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-16 |
| Detector | ADSC QUANTUM |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.533, 55.198, 46.751 |
| Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.900 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mix |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.230 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (CCP4) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.590 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.033 * | 0.163 * |
| Total number of observations | 36282 * | |
| Number of reflections | 13705 | |
| Completeness [%] | 91.5 * | 63.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 4 * | pH 7.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.0 |
| 2 | 1 | reservoir | PEG2000 MME | 20 (%(w/v)) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.0 |
| 5 | 1 | drop | 150 (mM) | ||
| 6 | 1 | drop | dithiothreitol | 2 (mM) |
