1MDU

Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1 (GS-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-03
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	67.187, 75.942, 96.747
Unit cell angles	90.00, 91.87, 90.00

Refinement procedure

Resolution	25.000 - 2.200
R-factor	0.207
Rwork	0.193
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1esv
RMSD bond length	0.012
RMSD bond angle	25.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.164
Number of reflections	45313
<I/σ(I)>	17	5.5
Completeness [%]	91.4	81.4 *
Redundancy	4. *	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	4 *	PEG 3350, tris, calcium chloride, magnesium chloride, ADP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG1000	15 (%(w/v))
3	1	reservoir	Tris-HCl	25 (mM)	pH8.0
4	1	reservoir	ATP	2 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir		2 (mM)