1MAS

PURINE NUCLEOSIDE HYDROLASE

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1995-01-24
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	63.840, 131.530, 90.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.000 - 2.500
R-factor	0.17 *
Rwork	0.170
RMSD bond length	0.016
RMSD bond angle	1.510 *
Data reduction software	XENGEN (V. 2.1)
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.500
Rmerge	0.075 *
Number of reflections	25989
Completeness [%]	96.1
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	34 (mg/ml)
2	1	drop	phosphate	10 (mM)
3	1	drop	glycine	10 (mM)
4	1	reservoir	potassium citrate	100 (mM)
5	1	reservoir	PEG4000	8 (%)
6	1	reservoir	sodium azide	0.05 (%)