1M93

1.65 A Structure of Cleaved Viral Serpin CRMA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-12
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	49.520, 92.590, 100.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.540 - 1.650
Rwork	0.188
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1C8O
RMSD bond length	0.009
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.540	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.039	0.470
Number of reflections	53225
<I/σ(I)>	32.6	2.1
Completeness [%]	94.2	83.5
Redundancy	5.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium/potassium phosphate 1.6M, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K