1M8V
Structure of Pyrococcus abyssii Sm Protein in Complex with a Uridine Heptamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.000, 68.000, 84.800 |
| Unit cell angles | 105.00, 108.80, 100.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| Rwork | 0.212 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h64 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.650 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 37089 |
| <I/σ(I)> | 29.1 |
| Completeness [%] | 90.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG 1000, Imidazole pH8.0, Calcium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 4.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG1000 |
