1M7R

Crystal Structure of Myotubularin-related Protein-2 (MTMR2) Complexed with Phosphate

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-20
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	158.250, 82.660, 100.130
Unit cell angles	90.00, 117.93, 90.00

Refinement procedure

Resolution	8.000 - 2.600
Rwork	0.207
R-free	0.28700 *
Structure solution method	MAD
RMSD bond length	0.008 *
RMSD bond angle	1.330 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.760
High resolution limit [Å]	2.600	2.600
Rmerge	0.080 *	0.254 *
Total number of observations	459390 *
Number of reflections	39438
<I/σ(I)>	11.9	5.5
Completeness [%]	100.0	100 *
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	PEG 35000, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 100.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.0 (mg/ml)
2	1	drop	Tris-HCl	7 (mM)	pH8.0
3	1	drop		15 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	reservoir	HEPES	100 (mM)	pH7.5
6	1	reservoir	TCEP	2 (mM)
7	1	reservoir		10 (mM)
8	1	reservoir	PEG35000	2.75 (%)