1M6V
Crystal Structure of the G359F (small subunit) Point Mutant of Carbamoyl Phosphate Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-12-11 |
| Detector | SBC-2 |
| Wavelength(s) | 0.70004 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 151.500, 164.200, 331.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.18 |
| Rwork | 0.174 |
| R-free | 0.24500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jdb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.330 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.076 * | 0.244 * |
| Number of reflections | 445772 | 40543 * |
| <I/σ(I)> | 16.2 | 2.7 |
| Completeness [%] | 92.1 | 84.4 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.4 | 4 * | PEG-8000, manganese chloride, potassium chloride, ADP, ornithine, tetraethylammonium chloride, HEPPS, pH 7.4, batch, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 8 (%) | |
| 2 | 1 | 1 | TEA | 0.65 (M) | |
| 3 | 1 | 1 | 0.5 (mM) | ||
| 4 | 1 | 1 | 100 (mM) | ||
| 5 | 1 | 1 | ADP | 1.5 (mM) | |
| 6 | 1 | 1 | L-ormithine | 0.5 (mM) | |
| 7 | 1 | 1 | HEPPS | 25 (mM) | pH7.4 |
