1M4B
Crystal Structure of Human Interleukin-2 K43C Covalently Modified at C43 with 2-[2-(2-Cyclohexyl-2-guanidino-acetylamino)-acetylamino]-N-(3-mercapto-propyl)-propionamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.488, 35.102, 36.642 |
| Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.150 |
| R-factor | 0.24686 |
| Rwork | 0.245 |
| R-free | 0.28584 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.771 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.210 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.067 | 0.340 |
| Number of reflections | 6926 | |
| <I/σ(I)> | 12.7 | 6.7 |
| Completeness [%] | 97.5 | 78 |
| Redundancy | 4.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
