1M2T
Mistletoe Lectin I from Viscum album in Complex with Adenine Monophosphate. Crystal Structure at 1.9 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-09-29 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8459 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 107.120, 107.120, 309.820 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 1.890 |
R-factor | 0.2101 |
Rwork | 0.210 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | unpublished structure |
RMSD bond length | 0.012 |
RMSD bond angle | 0.029 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.930 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.068 | 0.764 |
Number of reflections | 83270 | |
<I/σ(I)> | 22 | 2.3 |
Completeness [%] | 100.0 | 99.5 |
Redundancy | 38 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion under microgravity | 2.5 | 293 | ammonium sulphate, pH 2.5, vapor diffusion under microgravity at 293K |