1M2G

Sir2 homologue-ADP ribose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	90
Detector technology	IMAGE PLATE
Detector	MAC Science DIP-2030
Spacegroup name	P 1 21 1
Unit cell lengths	35.917, 85.512, 44.854
Unit cell angles	90.00, 112.34, 90.00

Refinement procedure

Resolution	40.000 * - 1.470 *
R-factor	0.189
Rwork	0.189
R-free	0.23100 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.160 *
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	1.470 *
Rmerge	0.031 *
Total number of observations	98112 *
Number of reflections	36883 *
Completeness [%]	88.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	18 *	Sodium Citrate, PEG 4000, pH 6.0, EVAPORATION, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mM)
2	1	reservoir	sodium citrate	100 (mM)	pH6.0
3	1	reservoir	PEG4000	14 (%)
4	1	reservoir	isopropanol	20 (%)