1M1M
X-RAY CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS BETA-KETOACYL-ACYL CARRIER PROTEIN SYNTHASE III (MTFABH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-27 |
| Detector | MACSCIENCE DIP100 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.313, 80.909, 96.334 |
| Unit cell angles | 90.00, 106.08, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.700 |
| Rwork | 0.212 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.650 * | |
| Rmerge | 0.068 * | 0.378 * |
| Total number of observations | 51681 * | |
| Number of reflections | 21140 * | |
| Completeness [%] | 97.0 * | 97.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 297 | Sodium formate, pH 7.5, Microbatch, temperature 297K |
