1M1L

Human Suppressor of Fused (N-terminal domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	173.025, 173.025, 290.833
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.810 - 2.650
Rwork	0.224
R-free	0.25600 *
Structure solution method	SAD
RMSD bond length	0.010 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000 *
High resolution limit [Å]	2.650	2.650 *
Rmerge	0.094 *	0.362 *
Total number of observations	441505 *
Number of reflections	48643
Completeness [%]	99.2	100 *
Redundancy	9.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	279	1 M LiCl, 0.1 M NaCitrate pH 5.0- 6.0, 10% w/v PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 279K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5 (mg/ml)
2	1	drop	Tris	25 (mM)	pH7.5
3	1	drop	dithiothreitol	10 (mM)
4	1	reservoir		1 (M)
5	1	reservoir	sodium citrate	0.1 (M)	pH5.0-6.0
6	1	reservoir	PEG6000	10 (%(w/v))