1M10
Crystal structure of the complex of Glycoprotein Ib alpha and the von Willebrand Factor A1 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8075 |
| Spacegroup name | P 61 |
| Unit cell lengths | 89.840, 89.840, 124.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.500 - 3.100 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1AUQ and 1M0Z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 3.200 |
| High resolution limit [Å] | 3.100 * | 3.100 * |
| Rmerge | 0.087 | 0.480 |
| Number of reflections | 10454 | |
| <I/σ(I)> | 19.3 | 3.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 4 * | PEG 3000, sodium chloride, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 25 (mM) | |
| 2 | 1 | drop | 100 (mM) | pH7.8 | |
| 3 | 1 | drop | protein | 4 (mg/ml) | |
| 4 | 1 | reservoir | PEG3000 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | 200 (mM) | ||
| 6 | 1 | reservoir | MES | 100 (mM) | pH5.5 |
