1M0I

Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.978
Spacegroup name	P 21 21 2
Unit cell lengths	123.440, 134.550, 61.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.130 - 2.550
R-factor	0.222
Rwork	0.222
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m0d
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.130	2.680
High resolution limit [Å]	2.550	2.550
Number of reflections	34014
<I/σ(I)>	9.5	2.4
Completeness [%]	99.8	99.9
Redundancy	4.9	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop. Seeds of E65K mutant used.	7.2	291	PEG 4000, Ammonium sulphate, Sodium chloride, Tris HCL, pH 7.2, Vapor diffusion, hanging drop. Seeds of E65K mutant used., temperature 291K