1LZV

Site-Specific Mutant (Tyr7 replaced with His) of Human Carbonic Anhydrase II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2002-04-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.548
Spacegroup name	P 1 21 1
Unit cell lengths	42.871, 41.585, 72.951
Unit cell angles	90.00, 104.75, 90.00

Refinement procedure

Resolution	19.940 - 2.300
Rwork	0.186
R-free	0.24800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g0f
RMSD bond length	0.006
RMSD bond angle	1.366 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.151	0.401 *
Number of reflections	9586 *	979 *
Completeness [%]	85.0	87.3 *
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	40 *	Ammonium Sulfate, Tris HCL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.8
3	1	reservoir	ammonium sulfate	2.4-2.65 (M)
4	1	reservoir	Tris-HCl	50 (mM)	pH7.8