1LYA

CRYSTAL STRUCTURES OF NATIVE AND INHIBITED FORMS OF HUMAN CATHEPSIN D: IMPLICATIONS FOR LYSOSOMAL TARGETING AND DRUG DESIGN

Experimental procedure

Spacegroup name	P 65
Unit cell lengths	125.900, 125.900, 104.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.188
Rwork	0.188
RMSD bond length	0.012
RMSD bond angle	3.100
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.1 *	20 *	taken from Gulnik, S. et al (1992). J. Mol. Biol., 227, 265-270. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20 (mg/ml)
2	1	reservoir	sodium acetate	50 (mM)
3	1	reservoir	ammonium sulfate	65 (%sat)