1LX5
Crystal Structure of the BMP7/ActRII Extracellular Domain Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 140.920, 140.920, 90.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 * - 3.300 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.27900 |
| Structure solution method | MIR |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.978 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 * | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.055 * | 0.332 * |
| Total number of observations | 31531 * | |
| Number of reflections | 8320 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 96.7 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 23 * | 1M sodium acetate, 0.1M imidazole, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 1 (M) | |
| 3 | 1 | reservoir | imidazole | 100 (mM) | pH7. |
