1LWK

Multiple Methionine Substitutions are Tolerated in T4 Lysozyme and have Coupled Effects on Folding and Stability

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	2002-02-10
Detector	MARRESEARCH
Wavelength(s)	0.82653
Spacegroup name	P 32 2 1
Unit cell lengths	60.332, 60.332, 91.367
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.000 - 2.100
R-factor	0.219
Rwork	0.217
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.018
RMSD bond angle	2.900
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	11279
Completeness [%]	99.2	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP