1LVW

Crystal structure of glucose-1-phosphate thymidylyltransferase, RmlA, complex with dTDP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-04
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0719, 1.0726, 1.0455
Spacegroup name	P 21 21 21
Unit cell lengths	112.680, 115.880, 116.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.520 - 1.700
Rwork	0.187
R-free	0.21500
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	161361
<I/σ(I)>	19.4	2.5
Completeness [%]	96.2	71.6
Redundancy	4.15	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	2.0 M ammonium sulfate, 0.1 M sodium acetate, 24% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K