1LVC
Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin and 2' deoxy, 3' anthraniloyl ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 116.924, 167.918, 341.743 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.960 - 3.600 |
| R-factor | 0.281 |
| Rwork | 0.281 |
| R-free | 0.30700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1k90 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.960 | 3.730 |
| High resolution limit [Å] | 3.600 * | 3.600 |
| Rmerge | 0.083 | 0.356 |
| Number of reflections | 38841 | |
| <I/σ(I)> | 26 | 10 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 6.85 * | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 298 | Hiratsuka, T., (1983) Biochem. Biophys. Acta., 742, 496. * |
