1LV0

Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	98
Detector technology	CCD
Collection date	2000-02-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	62.230, 42.861, 89.104
Unit cell angles	90.00, 103.53, 90.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.196
Rwork	0.196
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d5t
RMSD bond length	0.005 *
RMSD bond angle	23.800 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.061	0.419
Total number of observations	68840 *
Number of reflections	30213
<I/σ(I)>	12.7	1.4
Completeness [%]	95.7	85.9
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.25 *	22.5 *	Luan, P., (2000) Traffic, 1, 270., Schalk, I., (1996) Nature,381, 42. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.73 (M)