1LTX

Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 1 21 1
Unit cell lengths	68.700, 197.300, 85.300
Unit cell angles	90.00, 112.80, 90.00

Refinement procedure

Resolution	19.740 - 2.800 *
R-factor	0.224
Rwork	0.224
R-free	0.27400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dce
RMSD bond length	0.007 *
RMSD bond angle	1.280 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.094 *	0.364 *
Number of reflections	54362	3716 *
<I/σ(I)>	9.5	3.25
Completeness [%]	94.5	62.5
Redundancy	3.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.2	298	used microseeding, Rak, A., (2001) J.STRUCT.BIOL., 136, 158. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG3350	20 (%)
3	1	reservoir	KSCN	100 (mM)
4	1	reservoir	Namalonate	100 (mM)