1LQS

CRYSTAL STRUCTURE OF HUMAN CYTOMEGALOVIRUS IL-10 BOUND TO SOLUBLE HUMAN IL-10R1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-10-30
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	114.290, 104.680, 91.890
Unit cell angles	90.00, 106.49, 90.00

Refinement procedure

Resolution	50.000 - 2.700
Rwork	0.244
R-free	0.29500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j7v
RMSD bond length	0.007 *
RMSD bond angle	1.350 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.056 *	0.195
Total number of observations	65855 *
Number of reflections	26564 *
Completeness [%]	92.5 *	73.7 *
Redundancy	2.8 *	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	25 *	PEG-6000, 0.1M MgCl2, 100mM ADA, 0.75% PEG-400, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	20 (mM)	pH8.0
3	1	reservoir	ADA buffer	100 (mM)	pH6.0
4	1	reservoir	PEG6000	6 (%)
5	1	reservoir		0.1 (M)
6	1	reservoir	PEG400	0.75 (%)