1LPH

LYS(B28)PRO(B29)-HUMAN INSULIN

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-06-09
Detector	RIGAKU RAXIS IIC
Spacegroup name	H 3
Unit cell lengths	79.620, 79.620, 37.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.161
Rwork	0.161
RMSD bond length	0.016 *
RMSD bond angle	0.054 *
Data reduction software	RIGAKU
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.500
High resolution limit [Å]	2.300
Rmerge	0.060
Total number of observations	19299 *
Number of reflections	3910
Completeness [%]	83.4
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	human insulin	0.27 (mM)
2	1	1	hydrochloric acid	0.12 (M)
3	1	1	sodium citrate	0.05 (M)