1LOS

crystal structure of orotidine monophosphate decarboxylase mutant deltaR203A complexed with 6-azaUMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	52.490, 56.152, 73.870
Unit cell angles	82.76, 89.89, 76.91

Refinement procedure

Resolution	30.000 * - 1.900
R-factor	0.205
Rwork	0.205
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	22.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.048 *	0.283 *
Total number of observations	489020 *
Number of reflections	58208
Completeness [%]	90.6	64

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	trisodium citrate, MgCl2, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		25 (mM)
2	1	reservoir	dioxane	5 (%)