1LMK

THE STRUCTURE OF A BIVALENT DIABODY

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	72.140, 80.710, 88.010
Unit cell angles	90.00, 99.81, 90.00

Refinement procedure

Resolution	6.000 - 2.600
R-factor	0.2
Rwork	0.200
RMSD bond length	0.018
RMSD bond angle	2.400
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.125 *
Total number of observations	124443 *
Number of reflections	31023
Completeness [%]	98.4
Redundancy	4. *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.8 *	17 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein
2	1	drop	glycerol	20 (%(v/v))	precipitant
3	1	drop	PEG3000	15 (%(v/v))	precipitant
4	1	drop	Tris-HCl	0.1 (M)	precipitant
5	1	drop	sodium acetate	0.2 (M)	precipitant
6	1	reservoir	precipitant