1LM5

Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.925
Spacegroup name	P 21 21 21
Unit cell lengths	68.320, 95.379, 54.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 * - 1.800
R-factor	0.203
Rwork	0.203
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.420 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000 *	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.031 *	0.300 *
Total number of observations	292583 *
Number of reflections	32732 *
Completeness [%]	96.1	90.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	20 *	PEG 4000, magnesium chloride, acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	sodium acetate	100 (mM)	pH5.2
3	1	reservoir	PEG4000	31 (%(w/v))
4	1	reservoir		150 (mM)