1LLM
Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 133 |
Detector technology | IMAGE PLATE |
Collection date | 2000-06-08 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 87.398, 87.398, 118.693 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
Rwork | 0.216 |
R-free | 0.23400 |
Structure solution method | MIR |
RMSD bond length | 0.015 |
RMSD bond angle | 1.640 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.095 * | |
Total number of observations | 1187929 * | |
Number of reflections | 83074 * | |
Completeness [%] | 98.9 * | 97.9 |
Redundancy | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | bis-tris propane, magnesium chloride, ammonium acetate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | bis-tris propane, magnesium chloride, ammonium acetate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | bis-tris propane | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | ammonium acetate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1.9 (mM) | |
2 | 1 | drop | Bis-Tris propane | 80 (mM) | pH8.0 |
3 | 1 | drop | DNA | 0.056 (mM) | |
4 | 1 | drop | Bis-Tris propane | 10 (mM) | pH8.0 |
5 | 1 | drop | 1 (mM) | ||
6 | 1 | reservoir | 600 (mM) |