1LL0

Crystal Structure of Rabbit Muscle Glycogenin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-01
Detector	SBC-2
Wavelength(s)	0.9795, 0.9794, 0.9322
Spacegroup name	P 43 21 2
Unit cell lengths	139.460, 139.460, 416.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 3.430
R-factor	0.252 *
Rwork	0.252
R-free	0.28600 *
Structure solution method	MAD
RMSD bond length	0.011 *
RMSD bond angle	1.450 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.520
High resolution limit [Å]	3.430	3.430
Rmerge	0.088	0.484
Total number of observations	403861 *
Number of reflections	56152
<I/σ(I)>	26	4.2
Completeness [%]	99.6	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	4 *	8-10 mg/mL glycogenin, 0.7 to 1.2 M ammonium sulphate, and 100 mM sodium phosphate buffer pH 6.6 to 6.9, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	glycogenin	8-10 (mg/ml)
2	1	reservoir	ammonium sulfate	0.7-1.2 (M)
3	1	reservoir	sodium phosphate	100 (mM)	pH6.6-6.9