1LKY

Structure of the wild-type TEL-SAM polymer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2001-04-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	52.752, 60.291, 62.318
Unit cell angles	116.21, 98.89, 98.65

Refinement procedure

Resolution	500.000 * - 2.300
R-factor	0.23 *
Rwork	0.232
R-free	0.27200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ji7
RMSD bond length	0.006
RMSD bond angle	1.140 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000 *	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.087	0.335 *
Number of reflections	53910
Completeness [%]	97.5	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	5% PEG 4000 2.0 M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	Bis-Tris propane	10 (mM)	pH8.5
3	1	drop		200 (mM)
4	1	reservoir	PEG4000	5 (%)
5	1	reservoir	ammonium sulfate	2.0 (M)