1LJM
DNA recognition is mediated by conformational transition and by DNA bending
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-18 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 121.230, 121.230, 186.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.000 - 2.500 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e50 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MTF |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.071 | 0.295 |
| Total number of observations | 243124 * | |
| Number of reflections | 15686 * | |
| <I/σ(I)> | 17.2 | 2.7 |
| Completeness [%] | 99.3 | 99.8 * |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | Ethanol, Sodium Chloride, Tris-HCl, Hepes, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8-12 (mg/ml) | |
| 2 | 1 | reservoir | ethanol | 6 (%) | |
| 3 | 1 | reservoir | 1.4-1.5 (M) | ||
| 4 | 1 | reservoir | Tris | 100 (mM) | pH7.5 |
| 5 | 1 | reservoir | dithiothreitol | 20 (mM) |
