1LJM

DNA recognition is mediated by conformational transition and by DNA bending

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-01-18
Detector	RIGAKU RAXIS IV
Spacegroup name	H 3 2
Unit cell lengths	121.230, 121.230, 186.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.000 - 2.500
R-factor	0.1947
Rwork	0.193
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e50
RMSD bond length	0.007
RMSD bond angle	26.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MTF
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.071	0.295
Total number of observations	243124 *
Number of reflections	15686 *
<I/σ(I)>	17.2	2.7
Completeness [%]	99.3	99.8 *
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	Ethanol, Sodium Chloride, Tris-HCl, Hepes, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8-12 (mg/ml)
2	1	reservoir	ethanol	6 (%)
3	1	reservoir		1.4-1.5 (M)
4	1	reservoir	Tris	100 (mM)	pH7.5
5	1	reservoir	dithiothreitol	20 (mM)