1LB8

Crystal structure of the Non-desensitizing GluR2 ligand binding core mutant (S1S2J-L483Y) in complex with AMPA at 2.3 resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2001-05-30
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 2
Unit cell lengths	98.560, 121.376, 47.342
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.520 - 2.300
Rwork	0.218
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftm
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	25961
Completeness [%]	99.6	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	PEG 8000, ammonium sulfate, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K