1LAT
GLUCOCORTICOID RECEPTOR MUTANT/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-08-16 |
| Detector | FUJI |
| Wavelength(s) | 0.908 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.720, 76.070, 118.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.195 * |
| Rwork | 0.195 |
| R-free | 0.28100 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 6.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.060 |
| Number of reflections | 26580 |
| Completeness [%] | 96.6 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | ETHANOL | ||
| 3 | 1 | 1 | CACODYLATE | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | MG SULFATE | ||
| 6 | 1 | 1 | ZNCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | E/TRGR-GRES0 complex | 0.1 (mM) | |
| 2 | 1 | drop | Na cacodylate | 0.02 (M) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 8 (mM) | ||
| 5 | 1 | drop | 2 (mM) | ||
| 6 | 1 | reservoir | ethanol | 7 (%) | |
| 7 | 1 | reservoir | Na cacodylate | 36 (mM) | |
| 8 | 1 | reservoir | 200 (mM) |
