1LAM

LEUCINE AMINOPEPTIDASE (UNLIGATED)

Experimental procedure

Temperature [K]	123
Detector technology	AREA DETECTOR
Collection date	1995-06-01
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	P 63 2 2
Unit cell lengths	131.500, 131.500, 121.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 1.600
R-factor	0.172
Rwork	0.172
R-free	0.20400
RMSD bond length	0.011
RMSD bond angle	1.800 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.600 *
Rmerge	0.070
Total number of observations	357105 *
Number of reflections	76376
Completeness [%]	94.4
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8		pH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	b1LAP	7 (mg/ml)
2	1	drop		0.050 (mM)
3	1	drop		200 (mM)
4	1	drop	Tris-HCl	50 (mM)
5	1	reservoir	Tris-HCl	50 (mM)
6	1	reservoir		0.050 (mM)