1L4D
CRYSTAL STRUCTURE OF MICROPLASMINOGEN-STREPTOKINASE ALPHA DOMAIN COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.449, 94.177, 126.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| Rwork | 0.206 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ddj |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.730 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.140 | 0.470 * |
| Number of reflections | 16982 * | 1167 * |
| <I/σ(I)> | 8.48 | 1.6 |
| Completeness [%] | 92.4 | 64.7 |
| Redundancy | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | Li2SO4, Hepes, Mg Acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | SKalpha | 18 (mg/ml) | |
| 10 | 1 | reservoir | magnesium acetate | 10 (mM) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | drop | urea | 0.4 (M) | |
| 4 | 1 | drop | uPgS741A | 16 (mg/ml) | |
| 5 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 6 | 1 | drop | urea | 0.4 (M) | |
| 7 | 1 | drop | NaN3 | 0.02 (%(w/v)) | |
| 8 | 1 | reservoir | 1.0 (M) | ||
| 9 | 1 | reservoir | HEPES | 50 (mM) | pH7.0 |
