1L3L
Crystal structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-12 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795, 0.9797, 0.95600 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.298, 58.370, 127.453 |
| Unit cell angles | 90.00, 102.81, 90.00 |
Refinement procedure
| Resolution | 39.600 - 1.660 |
| Rwork | 0.246 |
| R-free | 0.25200 * |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 20.000 * |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.082 | 0.422 |
| Number of reflections | 139194 | |
| <I/σ(I)> | 21.76 | 2 |
| Completeness [%] | 89.1 | 70.9 |
| Redundancy | 6.69 | 5.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | Joachimiak, A., (1991) Methods Enzymol., 208, 82. * |
