1KZY

Crystal Structure of the 53bp1 BRCT Region Complexed to Tumor Suppressor P53

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	103
Detector technology	CCD
Collection date	1999-06-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.2832,1.2817,1.2622
Spacegroup name	P 21 21 21
Unit cell lengths	73.132, 94.976, 133.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.216 *
Rwork	0.216
R-free	0.25600
Structure solution method	MAD
RMSD bond length	0.014 *
RMSD bond angle	1.730 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	30624
Completeness [%]	91.7
Redundancy	16.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8 *	4 *	PEG4000, sodium citrate, ammonium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	50 (mM)	pH6.0
2	1	reservoir	dithiothreitol	5 (mM)
3	1	reservoir	PEG4000	16 (%(w/v))
4	1	drop	protein	13 (mg/ml)
5	1	drop	BTP-HCl	50 (mM)	pH6.8
6	1	drop		200 (mM)
7	1	drop	dithiothreitol	5 (mM)