1KZN

Crystal Structure of E. coli 24kDa Domain in Complex with Clorobiocin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	140
Detector technology	IMAGE PLATE
Collection date	1996-10-09
Wavelength(s)	0.938
Spacegroup name	P 21 21 21
Unit cell lengths	40.714, 47.629, 111.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.790 - 2.300
R-factor	0.239
Rwork	0.239
R-free	0.28000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	25.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.000	2.300
Rmerge	0.043 *
Number of reflections	16938
Completeness [%]	85.0	90
Redundancy	2.86 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	PEG 20000,propanol-2,HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	inhibitor	0.5 (mM)
3	1	reservoir	HEPES	100 (mM)	pH7.5
4	1	reservoir	PEG20000	24 (%)
5	1	reservoir	propanol-2	12 (%)