1KXG

The 2.0 Ang Resolution Structure of BLyS, B Lymphocyte Stimulator.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	108
Detector technology	CCD
Collection date	2000-07-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.942
Spacegroup name	P 65
Unit cell lengths	123.580, 123.580, 161.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 * - 2.000
R-factor	0.189 *
Rwork	0.189
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d0g
RMSD bond length	0.007
RMSD bond angle	1.300
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.080
High resolution limit [Å]	2.000	2.000
Rmerge	0.083 *	0.384 *
Total number of observations	1940104 *
Number of reflections	93234	9746 *
<I/σ(I)>	23.4	3.8
Completeness [%]	99.3	93.8
Redundancy	20.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	279	25% dioxane, 25 mM MgCl2, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 279.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium citrate	25 (mM)
3	1	drop		125 (mM)	pH6.
4	1	drop	dioxane	25 (%(v/v))
5	1	drop		25 (mM)
6	1	reservoir	dioxane	25 (%)
7	1	reservoir		25 (mM)