1KWR

HUMAN CARBONIC ANHYDRASE II COMPLEXED WITH INHIBITOR 0134-36

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2000-05-18
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	42.560, 41.400, 72.340
Unit cell angles	90.00, 104.40, 90.00

Refinement procedure

Resolution	10.000 - 2.300 *
Rwork	0.196
R-free	0.26900 *
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008 *
RMSD bond angle	1.443 *

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.300 *
Number of reflections	10124 *
Completeness [%]	95.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	6 *	tris, nacl, ammonium sulfate, MeHgAc, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.3 (mM)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		20 (mM)	pH8.0
4	1	reservoir	Tris-HCl	100 (mM)	pH8.5
5	1	reservoir		300 (mM)
6	1	reservoir	ammonium sulfate	27 (M)
7	1	reservoir		5 (mg)