1KSJ
Complex of Arl2 and PDE delta, Crystal Form 2 (SeMet)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.815, 65.726, 103.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 2.600 |
| R-factor | 0.214 |
| Rwork | 0.209 |
| R-free | 0.26400 |
| Structure solution method | combination of Molecular Replacement and SAD phasing |
| Starting model (for MR) | human Arf1-GppNHp N-terminally truncated by 17 residues (J. Goldberg Cell. 95:237-248 1998) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.650 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 9926 | |
| <I/σ(I)> | 25.1 | 7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 8.7 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 5000 MME, HEPES, MgCl2, beta mercaptoethanol, GTP, PEG 400 as cryo protectant, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
