1KQS
The Haloarcula marismortui 50S Complexed with a Pretranslocational Intermediate in Protein Synthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-29 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 212.784, 300.349, 574.961 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.1709 * |
| Rwork | 0.173 |
| R-free | 0.22050 * |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 1.333 |
| RMSD bond angle | 2.161 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.150 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Total number of observations | 2581688 * | |
| Number of reflections | 340617 * | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 277 | data unknown * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | NH4Cl | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | CdCl2 | ||
| 6 | 1 | 1 | POTASSIUM ACETATE | ||
| 7 | 1 | 1 | TRIS-MES |
