1KPS
Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9790, 0.9793, 0.9788, 0.9712 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.531, 126.509, 72.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.250 - 2.500 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.30000 |
| Structure solution method | 3 wavelength MAD dataset collected at NSLS beamline X4A to 2.8A |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 * | 0.340 * |
| Total number of observations | 274090 * | |
| Number of reflections | 27902 * | |
| Completeness [%] | 96.2 * | 92.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 291 | 2.0M ammonium phosphate, 0.1M hepes, 10mM CuCl2, 5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | pH8.0 |
| 4 | 1 | drop | BME | 1 (mM) | |
| 5 | 1 | reservoir | lithium sulfate | 1.0 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
| 7 | 1 | reservoir | sodium citrate | 50 (mM) | pH5.5 |
| 8 | 1 | reservoir | glycerol | 5 (%) |
