1KO0
Crystal Structure of a D,L-lysine complex of diaminopimelate decarboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 98.997, 98.997, 176.966 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
Rwork | 0.181 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1knw |
RMSD bond length | 0.014 |
RMSD bond angle | 0.014 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.170 |
Rmerge | 0.073 |
Completeness [%] | 97.8 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | Lithium sulfate, MES, pyridoxal-5'-phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |