1KNU
LIGAND BINDING DOMAIN OF THE HUMAN PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA IN COMPLEX WITH A SYNTHETIC AGONIST
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0292 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.270, 63.440, 119.240 |
| Unit cell angles | 90.00, 101.84, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.500 |
| Rwork | 0.224 |
| R-free | 0.26400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1prg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNX (2000) |
| Refinement software | CNX (2000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.076 | 0.343 |
| Number of reflections | 22943 | |
| <I/σ(I)> | 13.05 | 3.49 |
| Completeness [%] | 97.3 | 98.2 * |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | 294 | CITRIC ACID, TRIS-HCL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium citrate | 0.8 (M) | |
| 2 | 1 | 1 | Tris | 0.15 (M) | pH8.0 |
| 3 | 1 | 1 | protein | 5 (mg/ml) |
