1KNO
CRYSTAL STRUCTURE OF THE COMPLEX OF A CATALYTIC ANTIBODY FAB WITH A TRANSITION STATE ANALOG: STRUCTURAL SIMILARITIES IN ESTERASE-LIKE ABZYMES
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.901 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.100, 76.900, 88.300 |
| Unit cell angles | 93.80, 93.90, 115.60 |
Refinement procedure
| Resolution | 7.000 - 3.200 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.27200 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 27.800 * |
| Data reduction software | MOSFLM (5.2) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 3.200 | 3.200 * |
| Rmerge | 0.112 | |
| Number of reflections | 27401 | |
| Completeness [%] | 96.0 | 89 * |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | 0.5 (mM) | ||
| 11 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 12 | 1 | reservoir | 0.05 (%) | ||
| 13 | 1 | reservoir | 0.15 (M) | ||
| 2 | 1 | drop | PEG10000 | 8 (%(w/v)) | |
| 3 | 1 | drop | glycerol | 17 (%(v/v)) | |
| 4 | 1 | drop | 0.5 (mM) | ||
| 5 | 1 | drop | hapten 3 | 1 (mM) | |
| 6 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 7 | 1 | drop | 0.05 (%) | ||
| 8 | 1 | reservoir | PEG10000 | 8 (%(w/v)) | |
| 9 | 1 | reservoir | glycerol | 17 (%(v/v)) |
